| Milepost | Location |
|---|---|
| Maps | |
| Schedules | |
| 81.3 | Louis |
| 88.9 | Iola |
| 95.6 | Edgewood |
| 101 | Gilmore |
| 106.7 | Altamont |
| 113 | Moccasin |
| 115.9 | Beecher City |
| 121.9 | Cowden |
| 133.1 | Tower Hill |
| 139.6 | Pana |
| 143.4 | Millersville |
| 148.3 | Owaneco |
| 155.7 | Taylorville |
| 161.0 | Sharpsburg |
| 164.6 | Edinburg |
| 168.9 | Breckenridge |
| 174.8 | Rochester |
| 178.3 | Sangamon Junction |
| 179.9 | Tansey Tower |
| 181.4 | Avenue Tower |
| 181.9 | WR Tower |
| 182.3 | B and O/IC Union Station |
| 182.4 | Fourth Street |
| 182.5 | JA Tower C and A Crossing |
| 182.6 | Second Street |
| 187.7 | Bradfordton |
| 194.6 | Richland |
| 198.4 | Pleasant Plains |
| 203.0 | Ashland |
| 209.6 | Philadelphia |
| 215.1 | Virginia |
| 223.0 | Bluff Springs |
| 228.2 | Beardstown |